Recombinant horseradish peroxidase variants for targeted cancer treatment
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چکیده
منابع مشابه
Recombinant horseradish peroxidase variants for targeted cancer treatment
Cancer is a major cause of death. Common chemo- and radiation-therapies damage healthy tissue and cause painful side effects. The enzyme horseradish peroxidase (HRP) has been shown to activate the plant hormone indole-3-acetic acid (IAA) to a powerful anticancer agent in in vitro studies, but gene directed enzyme prodrug therapy (GDEPT) studies showed ambivalent results. Thus, HRP/IAA in antibo...
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Heme-containing plant peroxidases (EC 1.11.1.7) contain a highly conserved single tryptophan residue. Its replacement with Phe in recombinant horseradish peroxidase (rHRP) increased the stability of the mutant enzyme in acid media. The kinetic properties of native, wild-type, and W117F mutant recombinant horseradish peroxidase in the reactions of ammonium 2, 2;-azino-bis(3-ethylbenzthiazoline-6...
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Epidermal growth factor (EGF ) from mouse submaxillary glands is ;I polypeptide o f hi, 00.15 which stimulates cpithelial cell proliferation ;ind which inhibits gastric acid secretion both i t i iii.o I I I a n d i r i ~ i i r o 12. 3 I. Inhibition o f pepsinogen secretion b y k<iF has been observed i t i t i i v 14 I. but this effect could be secondary t o an inhibition of acid secretion. T h ...
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The present study explains computational methods to design thermostable horseradish peroxidase enzyme using the crystal structure available from Protein Data Bank (PDB ID: 6ATJ). Multiple mutations were introduced to the original enzyme and developed a model by using Modeler9.14. After designing the model functional effect was confirmed in terms of protein ligand binding by molecular docking us...
متن کاملCobalt-substituted horseradish peroxidase.
Horseradish peroxidase can be reconstituted with cobalt porphyrin to give a cobaltic holoenzyme having physicochemical properties quite similar to those of the native ferric protein. The cobaltic protein (Co3+HRP) can be reduced to the cobaltous form (CoHRP), the analogue of ferroperoxidase and the reduced cobalt protein can bind O2 to form an analogue of oxyferroperoxidase (Compound III). Sinc...
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ژورنال
عنوان ژورنال: Cancer Medicine
سال: 2016
ISSN: 2045-7634,2045-7634
DOI: 10.1002/cam4.668